The laccase is produced by submerged fermentation of Aspergillus oryzae followed by purification and formulation. Laccase catalyzes the oxidation of phenols and their derivatives, aromatic amines and their derivatives carboxylic acids and their derivatives, steroid hormones, metalloorganic compounds and some non-phenolic compounds, and the higher the redox potential of laccase, the wider the range of degradable substrates for it.
Laccase is a polyphenol
oxidase (ρ-dihydric
phenol oxidase, EC1.10.3.2) containing four copper ions, belonging to the
copper blue oxidase, present as a monomeric glycoprotein. Laccase exists in fungi,
bacteria and plants. It mainly takes effect on phenol-like compounds,
performing one-electron oxidation. It is capable of catalyzing the ring
cleavage reaction of aromatic compounds.