Neutral protease is produced by submerged fermentation of Bacillus subtilis followed by purification and formulation. It can catalyze the hydrolysis of protein to produce free amino acids and peptides at around neutral pH. Because of its fast hydrolysis under mild conditions and easy control, the product has been widely used in alcohol fermentation, leather softening and depilation, silk degumming, vegetable and animal protein hydrolysis, brewing, juice clarification, baking and feed, etc.